○ Evidence tier 5 — Replicated animal lifespan study

Record

DesignTransgenic genetic-clearance lifespan study (mouse)
DOI10.1038/nature16932
Citation statusdoi verified via Crossref 2026-07-12 (Nature 2016;530(7589):184-189, Baker et al.)

Five-qualifier claim

Species / populationINK-ATTAC transgenic wild-type mice, two genetic backgrounds.
Exposure, route, scheduleAP20187 twice weekly from ~1 year of age to clear p16-expressing cells.
Comparator / durationVehicle-treated transgenic controls; followed for lifespan.
Endpoint / numeric resultLifelong clearance of p16-positive cells extended median lifespan in both sexes and delayed tumorigenesis and organ deterioration, without overt side effects.
What it did NOT establishMedian-lifespan extension via a genetic tool in mice; does not establish any human drug effect.

Primary reference

https://www.nature.com/articles/nature16932

Exact identifier to be confirmed against the primary record before publication where flagged above.